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Expression of variants of the transmembrane glycoprotein CD44 is known to correlate with disease progression and metastasis in a number of tumor types. We sought to better understand CD44 expression in mouse mammary carcinoma cells in vitro. C3H mammary carcinoma cells are derived from a DMBA-induced mammary carcinoma in a C3H/He mouse. C3(1)MC cells express CD44 variants exon 5 and 6 as the result of an N-methyl-N'-nitro-N-nitrosoguanidine-induced C3H-specific mutation. In this study, we found that all four variants of CD44 exon 7 were expressed at high levels in C3(1)MC cells, in contrast to the breast carcinoma cells used in this and a previous study. In addition, we found that all four exons of CD44 expressed in C3(1)MC cells were the result of N-methyl-N'-nitro-N-nitrosoguanidine-induced point mutations. Our results demonstrate that C3(1)MC cells contain an inducible genetic mutation which correlates with high levels of expression of CD44 variants. a “borrower” under the California Constitution.
We also note that a debtor does not automatically enjoy a right to discharge
based on the nature of the debt. See Vawter, 787 P.2d at 743 (“Although the Constitution
permits the legislature to declare a debt to be dischargeable, the legislature may still
declare it to be nondischargeable under any circumstances.”); Ewell v. 0b46394aab